Beilstein J. Org. Chem.2014,10, 1495–1503, doi:10.3762/bjoc.10.154
positioning of the pyrene units along the chain. An increase in triplex stability and a very high mismatch-selectivity, derived from combined stacking and base-pairing interactions, were found for PNA2, bearing two distant pyrene units.
Keywords: modified nucleobase; nucleic acids; PNA; pyrene excimer; SNP
recognition; triplex stabilization; Introduction
Peptide nucleic acid (PNA) probes are very selective in the recognition of DNA and have been used in a large variety of diagnostic methods, easily allowing the detection of point mutations at very low concentrations [1][2][3]. Poly-pyrimidine PNA can form very
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Graphical Abstract
Figure 1:
(a) TAT triplet structure showing Watson–Crick and Hoogsteen base pairing; the binding can be reinf...